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. Author manuscript; available in PMC: 2013 Feb 24.
Published in final edited form as: Exp Cell Res. 2012 May 29;318(16):2004–2013. doi: 10.1016/j.yexcr.2012.04.015

Fig. 3.

Fig. 3

Effects of inhibitors of MAP kinase, PKA, and CaM kinase on phosphorylation of ERK and CREB by Western blot. (A) UO126 (10 μM, U-400, Alamone Labs), a MAP kinase inhibitor, was used to treat the cells for 1 h before cells were treated with 500 nM OBDL for 5 min. (B) CaM kinase inhibitor (1 μM, 1277, Tocris), KN-62, was used to treat the cells for 1 h before cells were treated with 500 nM of OBDL for 5 min. (C) PKA inhibitor (0.1 μM, 1288, Tocris), KT 5720, was used to treat the cells for 1 h before cells were treated with 500 nM of OBDL for 5 min. Antibodies used in detecting p-ERK, ERK, p-CREB and CREB were used as previous experiments.