Figure 4.

Downregulation of ALG14 reduces surface expression of acetylcholine receptor in transfected HEK293 cells. (A) Silencing of ALG14 expressed in HEK 293 cells by small interfering RNAs (siRNA) targeted at ALG14 messenger RNA. Small interfering RNAs and the ALG14 expression construct were transfected into HEK 293 cells, and 48 h later, ALG14 expression analysed by western blot. (B) Two small interfering RNAs (1 and 10) targeted at ALG14 messenger RNA or ‘scrambled’ small interfering RNA were transfected into HEK 293 cells. Twenty-four hours later, the same cells were transfected with complementary DNAs encoding the human acetylcholine receptor α, β, δ and ε subunits, and 48 h later, cell-surface ¹²⁵I-α-bungarotoxin binding was determined (n = 3).