Table 2.
Ten genes validated by qRT-PCR and in situ hybridisation selected for morpholino knockdown. Fold changes are the means of both biological replicates. ♢ Indicates no reads in the gfp- samples, so a factor has been added to each read count prior to calculating a fold change (see Section 4 for details). EC: vascular endothelial cell; RBC: erythrocyte.
| Ensembl ID | Gene | Sequencing fold change | qRT-PCR fold change | In situ expression pattern |
|---|---|---|---|---|
| ENSDARG00000061747 | CC058 | 14.72♢ | 46.63 | EC, RBC |
| ENSDARG00000020031 | cldn11a | 14.80 | 59.79 | EC |
| ENSDARG00000041724 | glipr2 | 43.06 | 65.15 | EC, RBC |
| ENSDARG00000037883 | prcp | 6.15 | 18.93 | EC, RBC |
| ENSDARG00000063370 | sgk2a | 13.41 | 11.56 | EC, RBC |
| ENSDARG00000059682 | slc43a3 | 33.85 | 52.83 | EC, RBC |
| ENSDARG00000043604 | tmem205 | 18.47 | 53.79 | EC, RBC |
| ENSDARG00000056920 | tmem88a | 10.09 | 28.76 | EC, RBC |
| ENSDARG00000031817 | trim2a | 6.37 | 7.34 | EC, RBC |
| ENSDARG00000034453 | unc119a | 15.42 | 61.76 | EC, RBC |