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. 2013 Feb;53(2):139–151. doi: 10.1016/j.ceca.2012.11.005

Fig. 6.

Fig. 6

GSK-7975A impaired neither STIM1 oligomerization nor STIM1/Orai1 coupling. (A) Left: time course of relative FRET between CFP-STIM1 and YFP-STIM1 with or without perfusion of GSK-7975A. Right: localization, overlay and calculated FRET life cell image series of YFP-STIM1 and CFP-STIM1 (1) in the absence and (2) presence of GSK-7975A and (3) following store-depletion in the presence of GSK-7975A. (B) Left: time course of relative FRET between STIM1-CFP and Orai1-YFP upon store-depletion and after application of GSK-7975A. Right: localization, overlay and calculated FRET life cell image series of STIM1-CFP and Orai1-YFP (1) after store-depletion and (2) after perfusion of GSK-7975A.