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. 2013 Feb 25;3:1329. doi: 10.1038/srep01329

Figure 1. Let-7d is expressed in neural stem cells and down-regulates neural stem cell proliferation.

Figure 1

(a). Northern blot analysis of let-7d expression in different mouse tissues. U6 was used as the loading control. (b). Northern blot analysis of let-7d expression in mouse neural stem cells over 7 days of differentiation. Cells at day (d) 0 are the undifferentiated neural stem cells. U6 was used as the loading control. (c). BrdU labeling of neural stem cells transfected with let-7d. The RNA concentrations (from 0 to 200 nM) were indicated and BrdU labeling is shown in green. The merged images of BrdU staining and phase contrast are shown on the right. (d). Quantification of BrdU labeling in neural stem cells transfected with let-7d. The percentage of BrdU+ cells was scored by dividing the BrdU+ cells with total living cells. Error bars are standard deviation of the mean. * p<0.001 by one-way Anova test. (e). Let-7d transfection did not induce cytotoxicity. Neural stem cells were transfected with increasing concentrations of let-7d RNA duplexes, then analyzed for toxicity using LDH assays.