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. 2012 Jul 31;3(4):29. doi: 10.1186/scrt120

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Copyright ©2012 Ma et al.; licensee BioMed Central Ltd.

This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Human foreskin fibroblast supports self-renewal of mouse embryonic stem cells better than mouse embryonic fibroblast. (A) Alkaline phosphatase (AKP) staining of E14 mouse embryonic stem cells (ESCs) cultured on human foreskin fibroblast (HFF), mouse embryonic fibroblast (MEF) without exogenous leukemia inhibitory factor (LIF) or MEF with LIF for one, two and three passages, respectively. Scale bars: 100 μm. (B) The quantitative analysis of the results in (A). AKP-positive colonies in 10 (100×) microscopic fields were counted. The number of the AKP-positive colonies on HFF is set as 1.0. **P < 0.01, n = 3.