Table 1.
Forward and reverse primer sequences used for real-time PCR
| Species | Gene | Forward sequence | Reverse sequence |
| Human | ADAMTS-4 | 5'-CCCCAGACCCCGAAGAGCCA-3' | 5'-CCCGCTGCCAGGCACAGAAG-3' |
| Human | ADAMTS-5 | 5'-GGCCGTGGTGAAGGTGGTGG-3' | 5'-GCTGCGTGGAGGCCATCGTC-3' |
| Human | ACAN | 5'-ACCAGACGGGCCTCCCAGAC-3' | 5'-TGGCTCTGCCCCAGAGGGAC-3' |
| Human | COL21A | 5'-TGAGGGCGCGGTAGAGACCC-3' | 5'-TGCACACAGCTGCCAGCCTC-3' |
| Human | IL-1Ra (pair 1)* | 5'-CCCGTGAAGGAGAGCCCTTCATTTG-3' | 5'-ACTTTCACCATCATTTCACAAATGCAG-3' |
| Human | IL-1Ra (pair 2)** | 5'-TGTTCCATTCAGAGACGATCTGCCG-3' | 5'-GAGCATGAGGCTCAATGGGTACC-3' |
| Human | MMP-13 | 5'-ATGCGGGGTTCCTGATGTGG-3' | 5'-GGCCCAGGAGGAAAAGCATG-3' |
| Murine | Acan | 5'-CAGTGCGATGCAGGCTGGCT-3' | 5'-CCTCCGGCACTCGTTGGCTG-3' |
| Murine | Col2a1 | 5'-CACACTGGTAAGTGGGGCAAGACCG-3' | 5'-GGATTGTGTTGTTTCAGGGTTCGGG-3' |
| Murine | IL-1Ra | 5'-ACCCATGGCTTCAGAGGCAGC-3' | 5'-GCCCCCGTGGATGCCCAAG-3' |
*Primer pair used for IL-1Ra expression analysis in C28/I2 immortalized human chondrocytes and primary human chondrocytes for Figure 1A, 1C and Additional file 1. **Primer pair used for IL-1Ra expression analysis in primary human chondrocytes for Figure 4A and 4B. ACAN, aggrecan; COL2A1, type II collagen; IL-1Ra, interleukin-1 receptor antagonist; MMP-13, matrix metalloproteinase-13.