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. 2012 Jul 2;14(4):R157. doi: 10.1186/ar3897

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Copyright ©2012 Shirai et al.; licensee BioMed Central Ltd.

This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Inhibition test and epitope mapping. (A) Inhibition tests of binding activities to HUVECs were performed by using IgG from healthy donor (control) and anti-FLRT2 sera (X10-48) with soluble FLRT2 at the indicated concentrations. (B) Changes in binding activity to FLRT2 lacking the unique region (Δur) compared with native FLRT2 were analyzed by using anti-FLRT2 sera. Representative dot plot (left) and a summary of changes in each patient (right) are shown. Open circles show the binding activity to the intracellular FLRT2 domain.