Figure 3.

mRNA expression of angiogenesis-modulating factors during the course of collagen-induced arthritis (CIA). Mice at different stages of disease were sacrificed and whole paws processed for mRNA quantification by real-time RT-PCR. Data are presented as fold change in mRNA level relative to the mean expression in six age-matched healthy controls (dotted line) after normalisation to the acidic ribosomal phosphoprotein P0 (Arp) gene. Data are from individual mice (n = 5 to 7), bars indicate the median mRNA level of the different groups. Statistical analysis of data was performed using one-way ANOVA with Dunn's post hoc test for multiple comparisons, compared against pre-arthritic mice (*P < 0.05, **P < 0.01, ***P < 0.001).