Fig. (3).

Graphical representations of sequence conservation and CRE locations for Pax6 genes in C. intestinalis, mouse and amphioxus at the same genomic scale. Alignments were generated by AVID or LAGAN using the VISTA web application [43]. Experimentally verified CREs are denoted by colored overlays - green for enhancers, red for repressors, blue for CREs that modulate expression in some other way. Regions of expression for each CRE are noted. A) Plot of sequence conservation between C. intestinalis and C. savignyi for the ascidian Pax6 gene. B) Mouse vs. chicken. C) Amphioxus vs. Xenopus. No more conservation was seen in alignments of amphioxus with human, zebrafish, or lamprey DNA. (CREs: [44-47, 29]).