Fig. 2.

H₂O₂ is required for ABA-induced activation of ZmCPK11 in maize leaves. (A) Effect of pre-treatment with the ABA biosynthetic inhibitor fluridone (Flu) on the expression of ZmCPK11 in maize leaves exposed to PEG treatment. The fluridone-treated and -untreated seedlings were exposed to 10% PEG treatment for 1h. ABA (100 µM) was added to overcome the effects of fluridone. (B, C) Effects of pre-treatments with the ROS manipulators DMTU, DPI, and CAT on the expression of ZmCPK11 (B) and the activity of ZmCPK11 (C) in maize leaves exposed to ABA treatment. The detached maize plants were pre-treated with 10mM DMTU, 100 µM DPI, and 200U of CAT for 4h, and then exposed to 100 µM ABA for 30min (B) or 60min (C). Values are means ±SE of three independent experiments. Means denoted by the same letter did not significantly differ at P<0.05 according to Duncan’s multiple range test.