Fig. 6.

ZmCPK11 mediates ABA-induced production of H₂O₂ in maize protoplasts. (A) H₂O₂ fluorescence in protoplasts with transiently silenced ZmCPK11. The protoplasts were treated with 10 µM ABA (+ABA) or the incubation medium (–ABA) for 5min, and then loaded with H₂DCF-DA for 10min. CAT (20U) was also added to the control protoplasts in the presence or absence of ABA. The protoplasts transfected with water were used as controls. H₂O₂ was visualized by confocal microscopy. Experiments were repeated at least three times with similar results. (B) Changes in the fluorescence intensity in (A). Values are means ±SE of three independent experiments. Means denoted by the same letter did not significantly differ at P<0.05 according to Duncan’s multiple range test.