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. 2013 Jan 10;64(4):991–1003. doi: 10.1093/jxb/ers374

Fig. 5.

Fig. 5.

Overexpression of VvCEB1 strongly affects grape embryo development. (A) Relative expression level of VvCEB1 transcript accumulation in transgenic grape 41B embryos. The VvCEB1 transcript level was quantified by qRT-PCR in control (pFB8 empty vector) and VvCEB1-overexpressing (35S::VvCEB1) lines. These embryos were collected 7 d after initiation of embryogenesis. Gene expression was normalized against VvEF1γ. Data are means ±SD of three independent experiments. (B) Control (pFB8 empty vector) and VvCEB1-overexpressing (35S::VvCEB1) embryos lines were observed with a stereomicroscope 32 d after the initiation of embryogenesis. Bars, 1mm. (C) Embryos sizes were measured at different stages of 41B embryo development, from 7 to 32 d after initiation of embryogenesis (DAE), both for control (pFB8 empty vector, black circles) and VvCEB1-overexpressing (35S::VvCEB1, open circles) lines. Thirty embryos were measured for each developmental stage in both lines. Results are shown as means ±SD. Kruskal-Wallis one way analysis of variance on ranks was performed and asterisks indicate statistically significant differences between lines at the same time point (P<0.001).