Table 2.
Ratios of transcript levels of selected cell expansion-related genes in transgenic grape embryos compared with the control (C) and determination of the presence of bHLH-binding sites in the corresponding promoter regions.
| Description | Name | Accession no. | Ratio (35S/C)a | bHLH-binding sitesb |
|---|---|---|---|---|
| Expansin | VvEXPA1 | XM_002269481.1 | 7.7 | – |
| VvEXPA8 | XM_002280264.1 | 38 | + | |
| VvEXPA11 | XM_002285855.1 | 1154 | + | |
| VvEXPA12 | XM_002284822.1 | 52 | + | |
| VvEXPA17 | XM_002273247.1 | 252 | ++ | |
| α-Amylase | VvAMY1 | XM_002285177.1 | 4.3 | – |
| Xyloglucan endotransglycosylase | VvXET2 | XM_002274484.1 | 4 | ++ |
| Pectate lyase | VvPECL1 | XM_002285603.1 | 4.8 | ++++++++ |
| VvPECL8 | XM_002275745.1 | 82 | ++++++ | |
| Arabinogalactan protein | VvAGP20 | XM_002280458.1 | 5 | +++ |
| Aquaporin (TIP) | VvAQP1 | XM_002274502.1 | 8.3 | +++++ |
| VvAQP2 | XM_002262942.1 | 0.2 | + | |
| VvAQP3 | XM_002274691.1 | 4.1 | ++ | |
| VvAQP4 | XM_002274519.1 | 2.4 | + |
a Gene transcript levels were quantified by qRT-PCR in control (C, pFB8 empty vector) and VvCEB1-overexpressing (35S) grape 41B embryo lines. The embryos were collected 7 d after initiation of embryogenesis. Gene expression was normalized against VvEF1γ. Data are means of three independent experiments.
b The promoter regions (1000bp upstream of the transcription start site) for each gene were used as the target sequences for putative bHLH transcription factor recognition site identification using the MatInspector program (Genomatix software package). Each ‘+’ indicates the presence of one MYCL family binding site whereas ‘–’ indicates the absence of MYCL-binding site.