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. Author manuscript; available in PMC: 2013 Feb 25.
Published in final edited form as: J Mol Biol. 2010 Jun 25;401(3):350–362. doi: 10.1016/j.jmb.2010.06.041

Fig. 3.

Fig. 3

Binding of CcmE to CcmC requires heme. (a) A cartoon of the experimental design for the heme limitation results shown in (b) and (c). (b) Heme stains and anti-CcmE and anti-His Western blots of the elution (Elute) fractions of Ni2+-purified CcmC:6 × His with co-purified CcmE from −-ALA (lane 1) and +δ-ALA (lane 2) cultures. (c) Anti-CcmE Western blots of load and flow-through (FT) fractions from Ni2+-purified CcmC:6 × His experiments with co-purified CcmE from −δ-ALA (lanes 1 and 2) and +δ-ALA (lanes 3 and 4) cultures.