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. Author manuscript; available in PMC: 2013 Feb 25.
Published in final edited form as: Mol Cancer Res. 2011 Aug 17;9(10):1377–1384. doi: 10.1158/1541-7786.MCR-11-0198

Figure 4. PTP1B is not essential for tumor maintenance.

Figure 4

A. Immunoblotting of lysates from pools of MCF10A-NeuNT cells expressing a firefly control (CTRL) or PTP1B miR after 5 days of culture in the presence or absence of dox. Dox treatment of MCF10A-NeuNT cells deleted PTP1B (79.8%) in cells expressing PTP1B miR, but not in control cells.

B. PTP1B knockdown does not affect invasiveness of MCF10A-NeuNT cells. MCF10A-NeuNT cells expressing control or PTP1B miR were grown in 3D cultures in the presence or absence of dox. Phase contrast images showing the invasive structures.

C. Bar graph showing the mean percentage of MCF10A-NeuNT invasive structures ±SEM (n=3; P=0.85 student’s t-test).

D. Growth curves of MCF10A-NeuNT tumors in the presence or absence of PTP1B, showing the mean tumor volume (mm3) ±SEM (n=8; P=0.42 student’s t-test).

E. Immunoblot of lysates from MCF10A-NeuNT tumors in the presence or absence of PTP1B.