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. Author manuscript; available in PMC: 2014 Mar 1.
Published in final edited form as: Bioorg Med Chem Lett. 2012 Dec 20;23(5):1511–1518. doi: 10.1016/j.bmcl.2012.12.015

Figure 4. Incorporation of triphosphate of N3-d4U-6PEG-TIBO.

Figure 4

(A) Structure of triphosphate of N3-d4U-6PEG-TIBO (23). (B) Acrylamide gel showing incorporation of dTTP (100 μM) and triphosphate of N3-d4U-6PEG-TIBO (150 μM) over a time course under single turnover conditions (250 nM RT, 50 nM D23/D36 primer/template). Time points are t = 0, 30 sec, 5 min, 10 min, 30 min, and 60 min. Note the misincorporation of a second molecule of dTTP during this time course.