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. Author manuscript; available in PMC: 2014 Jan 22.
Published in final edited form as: Biochemistry. 2013 Jan 11;52(3):520–536. doi: 10.1021/bi301074r

Table 2.

Rate constants derived from Surface Plasmon Resonance (SPR) for full length baculovirus expressed phosphorylated SLBP towards SLIP1 at 25°C.

SLIP1 protein k+1 (M−1s−1) k−2 (s−1) KD (µM) KD (rel)*
Wild Type SLIP1 634 2.38E(-3) 3.75 ± 1.8
R202A SLIP1 n.d n.d n.d >100
K10A SLIP1 542 1.17E(-3) 2.15 ± 0.9 0.57
R76A SLIP1 181 5.49E(-4) 3.02 ± 2.2 0.81
E200A SLIP1 8310 5.49E(-3) .066 ± 0.02 .0176
H44A SLIP1 161 2.52E(-3) 15.6 ± 5.4 4.16
*

KD values representing KD (mut)/ KD (wt) i.e the interaction of mutant SLIP1 relative to the wild-type SLIP1 protein.