Figure 3.

Increased initial seeding density minimizes aggregate formation. A: mESCs were seeded onto Cultispher-S at a density of 6 × 10⁴ cells/mL using either (i) 2 min stirring at 15 rpm followed by 10 min off or (ii) 2 min stirring at 15 rpm followed by 30 min off. Samples were collected and the percentage of aggregates in the ranges indicated calculated for each condition. B: (i) The average number of cells remaining in the supernatant (n = 3, ±SD) and (ii) the total number of viable cells recoverable from each bioreactor (mean ± SD, n = 3) are shown.