Figure 3.

Torpedo nACh receptor current inhibition. Oocytes expressing wild type Torpedo nACh receptors were tested with 10 μM ACh (~EC₂₀) and then with 10 μM ACh plus increasing amounts of propofol or the propofol derivative. The current traces in the top right inset show one oocyte’s current response to 10 μM ACh plus increasing amounts of propofol (0.1, 0.3, 1, 3, 10, 30, 100, 300 μM). The effect of 6 on 10 μM ACh currents is shown in the bottom left inset (1, 3, 10, 30, 100 μM). Nonlinear least-squares analysis of the curves yielded: for propofol (open symbols), IC₅₀ = 7.3 ± 1.4 μM, nH = 0.8 ± 0.1 (3 oocytes); for 6 (solid symbols), IC₅₀ = 4.0 ± 1.2, nH = 0.9 ± 0.2 (3 oocytes). Currents were normalized to the 10 μM ACh response.