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. 2013 Feb 20;19:441–447.

Table 1. Primers and restriction enzymes used to genotype MMP polymorphisms.

SNP Forward primer C† PCR product R. E RFLP fragments Ref
MMP-1=(rs1799750)
c.-1607–1606insGG
TGACTTTTAAAACATAGTCTATGTTCA
58
269
AluI
1G/1G=241,28 2G/2G=269
[23]
TCTTGGATTGATTTGAGATAAGTCATAGA
MMP-7=(rs11568818) c.-181T>C
TGGTACCATAATGTCCTGAATG
65
150
EcoRI
T=150 C=120,30
[15]
TCGTTATTGGCAGGAAGCACACAATGAATT
MMP-9=(rs3918242) c.-1562C>T
GCCTGGCACATAGTAGGCCC
58
436
SphI
C=436 T=242,194
[24]
CTTCCTAGCCAGCCGGCATC
MMP-9=(rs17576) c.836A>G GAGAGATGGGATGAACTG
58 439 MspI A=252,187 G=187, 129, 123
  [25]
 
GTGGTGGAAATGTGGTGT

C†=annealing temperature; R.E=Restriction endonuclease