Table 1. Primers and restriction enzymes used to genotype MMP polymorphisms.
SNP | Forward primer | C† | PCR product | R. E | RFLP fragments | Ref |
---|---|---|---|---|---|---|
MMP-1=(rs1799750)
c.-1607–1606insGG |
TGACTTTTAAAACATAGTCTATGTTCA |
58 |
269 |
AluI |
1G/1G=241,28 2G/2G=269 |
[23] |
TCTTGGATTGATTTGAGATAAGTCATAGA | ||||||
MMP-7=(rs11568818) c.-181T>C |
TGGTACCATAATGTCCTGAATG |
65 |
150 |
EcoRI |
T=150 C=120,30 |
[15] |
TCGTTATTGGCAGGAAGCACACAATGAATT | ||||||
MMP-9=(rs3918242) c.-1562C>T |
GCCTGGCACATAGTAGGCCC |
58 |
436 |
SphI |
C=436 T=242,194 |
[24] |
CTTCCTAGCCAGCCGGCATC | ||||||
MMP-9=(rs17576) c.836A>G | GAGAGATGGGATGAACTG |
58 | 439 | MspI | A=252,187 G=187, 129, 123 | [25] |
GTGGTGGAAATGTGGTGT |
C†=annealing temperature; R.E=Restriction endonuclease