Skip to main content
. 2013 Feb 25;7:15. doi: 10.3389/fncir.2013.00015

Figure 3.

Figure 3

Monosynaptic retrograde tracing of inputs to new GCs. Virus I, Retroviral labeling. 1. Retrovirus (RV-SYN-GTRgp) expressing nucleus-localized histone-tagged green fluorescent protein (hGFP), avian TVA receptor, and rabies glycoprotein (gp) under control of the synapsin promoter infect proliferating neural progenitor cells (NPCs) in the DG. 2. The retrovirally labeled NPCs differentiate into newborn GCs over time and express hGFP, TVA and gp. Virus II, Rabies virus. 3. Avian envelope glycoprotein EnvA pseudotyped rabies virus, in which rabies gp was replaced with MCherry (EnvA-ΔG-MCh) is injected into the same DG at different time-points after retroviral labeling (21–90 dpi). Through interaction between EnvA glycoprotein and its receptor, TVA, pseudotyped rabies virus can selectively infect newborn GCs. 4. EnvA-ΔG-MCh rabies virus is complemented with rabies gp provided by the retrovirus and MCherry is expressed in the cytoplasm. 5. The rabies virus spreads trans-synaptically to presynaptic neurons connected to the new GC. 6. Only neurons synaptically connected are labeled and express MCherry. The traced cells lack rabies gp, therefore this virus will not spread any further.