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. 2013 Jan 14;24(2):170–180. doi: 10.1089/hum.2011.180

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Copyright 2013, Mary Ann Liebert, Inc.

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FIG. 4. — C-fos staining in the spinal cord. (A) Photomicrographs of c-fos staining in the dorsal horn of L6 spinal cord of a control rat (no treatment), a saline i.ves. rat (intravesical saline injection without virus treatment), a vHPPE RTx i.ves. rat (intravesical RTx injection after vHPPE treatment), and a vHG RTx i.ves. rat (intravesical RTx injection after vHG treatment). (B) The increase of c-fos–positive cells in the L6 dorsal horn after intravesical administration of 3 μM RTx was significantly lower in vHPPE-injected rats compared with vHG-injected rats or virus-untreated rats (*p<0.05). In control rats, only a low number of c-fos–positive cells were detected. (C) A schema showing the division of the dorsal horn into four regions: lateral dorsal horn (LDH), medial dorsal horn (MDH), dorsal commissure (DCM), and sacral parasympathetic nucleus (SPN). (D) The number of c-fos–positive cells in the four regions of the L6 dorsal horn in vHPPE-injected rats was lower in all four areas compared with vHG-injected rats, with significant differences in the SPN and DCM areas (*p<0.05). Columns and bars represent means±SEM. n.s., not significant.