FIG. 3.

DMPA treatment does not alter epithelial junction protein density. Immunolabeling for the epithelial tight junction protein E-cadherin (A–C) and adherens junction protein ZO-1 (D–F) in vaginal tissue biopsies obtained during the follicular (A, D) and luteal phases (B, E), and following administration of DMPA (C, F). No statistically significant difference in density was observed. Protein expression was calculated using the integrated optical density (IOD) in each area based on positive staining color.