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. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: Radiat Res. 2012 Feb 23;177(4):365–375. doi: 10.1667/rr2531.1

FIG. 3.

FIG. 3

Examples of functional data obtainable by intravital microscopy including the (panel A) bright field transmission image. Panel B: HIF-1 GFP reporter fluorescence (32), (panel C) total hemoglobin content, (panel D) hemoglobin oxygen saturation on a red-blue color scale (red: well oxygenated, blue: poorly oxygenated) whose brightness is modulated by the total hemoglobin content (making vessels appear bright), and (panel E) an overlay view of panels D and B with the GFP channel appearing in green. The tumor appears as the pickle-shaped object in the center of the view. The edge of the tumor can be seen to be highly-vascularized, with relatively few vessels in the hypoxic center of the tumor. Scale bars are 1 mm. (Reproduced with permission from ref. 63.)