Figure 3. .
Similar state of T cell activation in draining lymph nodes and post antigenic stimulation. A, Total cell numbers in two DLNs of WT and Aicda KO mice. B, T cell activation is normal in Aicda KO mice. DLN cells were stained with CD4, CD8, CD44 and CD62L and analyzed by flow cytometry. C) Aicda KO mice do not show defect in the MOG-specific T cell IL-17 production. WT and Aicda KO mice were immunized with MOG and 10 days later, draining lymph node cells from mice were isolated and cultured with various concentrations of rMOG or MOG35–55 peptide for 4 days and subsequently the supernatants were collected and analyzed by Luminex.