Figure 5. .

Surface plasmon resonance (SPR) analysis of serum antibody binding to MOG. A. SPR sensorgrams of serum samples binding to sensor immobilized MOG. Serum from 8 mice were pooled for each of the following sample sets: Day 0 WT, Day 0 KO, Day 28 WT, and Day 28 KO. Start and stop injection times are indicated by arrows. Day 28 WT serum showed the highest binding response, and the majority of this binding signal could be competed with soluble MOG (2 uM) preincubated with the serum sample before injection. B. Detection of the major binding isotype in Day 28 WT serum. Subsequent injections of three anti-isotype antibodies were used to probe the MOG bound component. Start and stop injection times are indicated by arrows for each sample (Day 28 WT serum, anti-mIgM, anti-mIgG2a and anti-mIgG1). The data indicate that the majority of the binding signal is specific to IgG1. No background binding of anti-mIgG1 to MOG alone was detected (data not shown).