FIG. 2.

The P0 promoter is selectively expressed in schwannoma cells. (A) 293T cells and HEI-193 schwannoma cells were infected with AAV1-CBA-GFP, AAV1-P0-GFP, or AAV1-P0-ICE vector in culture at a multiplicity of infection (MOI) of 10,000 GC/cell and, after 48 hr, were evaluated microscopically for GFP fluorescence and cell morphology at an original magnification of ×10. (B) Cell viability was assessed by Fluc bioluminescence in 293T, SH-SY5Y (neuroblastoma), and HEI-193 cells 72 hr after infection with AAV1-P0-ICE or AAV1-P0-GFP, or no infection, at the same MOI as in (A). Values for each cell line are normalized to viability of uninfected cells. *Significant difference compared with the control group (p=0.00006).