FIG. 5.

Neuropathological evaluation of sciatic nerve after AAV1-P0-ICE injection. Shown is toluidine blue staining of myelin in Epon-embedded sciatic nerve of nude mice (A–C) and immunocompetent C57BL/6 mice (D and E). Cross-sections of naive sciatic nerve (A), and 3 weeks (B and D), and 8 weeks (C and E) after the first of two weekly injection of AAV1-P0-ICE, show essentially normal nerves with rare axonal degeneration [(B), inset] and occasional macrophage infiltration [(C), inset] marked by arrows. Sciatic nerves of C57BL/6 mice (D and E) showed normal myelination and axonal integrity. Original magnification: (A)×20; (B–E): ×40; insets: ×100. Color images available online at www.liebertpub.com/hum