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Electrophoretic and chromatographic behaviors of CchGs. SDS–PAGE for CchGs with both heat treatment and mercaptoethanol (line 2), heat treatment but without mercaptoethanol (line 3), no heat treatment without mercaptoethanol (line 4). Standard protein bands (line 1) (A). Protein HPLC chart (B). Gel filtration chromatography analysis (C), and molecular size calibration for standard proteins. Molecular size estimated from the Kav value for CchG is indicated by arrow (D).
