. 2012 Dec 4;23(4):412–425. doi: 10.1093/glycob/cws165

Table V.

Amino acid residues of congerin I and galectin 1 that participate in intermolecular interactions^a with lactose and corresponding residues for CchG

Position	Congerin I	Galectin 1	CchG
Gal-O1	OW-Tyr51^b O		Trp50
Gal-O2	OW-Asp67^b O		Gly65^c/ASP65^d
	OW-Tyr51^b O		Trp50
Gal-O3	Arg29 N1		Arg28^e
	Arg29 N2		Arg28^e
	OW-Asp67^b O1		Gly65^c/ASP65^d
		OW-Asn46^b O1	Asn45
Gal-O4	His44 N2	His44 N2	Val43
	Arg29 N2		Arg28^e
	Arg48 N1	Arg48 N1	Arg47^e
		Asn46 O1	Asn45^e
Gal-C5	Trp70	Trp70	Trp68
Gal-O5	Arg48 N1	Arg48 N2	Arg47^e
Gal-O6	Asn61 N1	Asn61 N1	Asn60^e
	Glu73 O2	Glu73 O2	Glu71^e
		OW-Glu73^b O2	Glu71
Glc-O3	Arg48 N1	Arg48 N1	Arg47^e
	Arg48 N2	Arg48 N2	Arg47^e
	Glu73 O2	Glu73 O2	Glu71^e
	Arg75 N2	Arg75 N2	Arg73^e
Glc-O6	OW-Glu73^b O1		Glu71

^aAll interactions are hydrogen bonds except for Gal-C5 and Trp70.

^bIndirect hydrogen bond via a water molecule (OW).

^cFor CchGa∼d.

^dFor CchGe and f.

^eDirect hydrogen bond can be expected. Data for congerin I and galectin 1 were taken from Shirai et al., 1999.