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HPLC analysis of enzymatic hydrolysis product of C10HSL with the HSL-degrading enzyme from LPC029. The initial C10HSL (35 mM) was reacted with partially purified enzyme from LPC029 for 16 h and separated with HPLC. HPLC profiles of (a) HSL standard (3.5 mM); (b) unreacted C10HSL (35 mM); and (c) reaction product of C10HSL with LPC029 HSL-degrading enzyme. The product peak was eluted at 2.8 min. mAU is the abbreviation for milli-absorbance unit.
