Figure 8.

Differentiation potential of expanded ALDH^br cells. (a) Photomicrograph showing intracellular fat droplets in cells cultured in adipogenic medium. (b) Positive Oil Red-O staining of intracellular droplets. (c) Positive type-II collagen immunostaining (green) in cells cultured in chondrogenic medium. (d) Type-II collagen immunostaining of leg cartilage, as a positive control. (e) Positive sarcomeric α-actinin immunostaining (green) of an ALDH^br cell (the protein does not show clear sarcomeric distribution). (f) Corresponding negative control (secondary Ab only). (g) Positive Alizarin Red staining of expanded ALDH^br cells cultured in osteogenic medium.