Table 3.
Recovery of viable cells positive for cell marker per gram of tissue.
| Patient | SSEA4+ | LHR+ | VASA+ | ||||||
|---|---|---|---|---|---|---|---|---|---|
| Fresh cells | Frozen cells | Frozen tissue | Fresh cells | Frozen cells | Frozen tissue | Fresh cells | Frozen cells | Frozen tissue | |
| 1 | 382,500 | 133,392 | 126,144 | 459,000 | 222,571 | 523,697 | 10,128,600 | 2,894,214 | 5,642,505 |
| 2 | 642,000 | 271,081 | 540,099 | 1,883,200 | 378,955 | 5,003,870 | 8,089,200 | 5,820,763 | 24,075,066 |
| 3 | 215,200 | 141,845 | 215,335 | 1,102,900 | 67,546 | 1,069,848 | 10,733,100 | 1,939,856 | 11,733,700 |
| 4 | 829,602 | 259,954 | 108,069 | 3,402,562 | 1,201,910 | 644,282 | 28,455,915 | 11,992,882 | 6,595,656 |
| 5 | 1,085,311 | 426,618 | 599,564 | 1,141,806 | 1,865,490 | 3,814,563 | 25,813,056 | 15,991,324 | 6,356,519 |
|
| |||||||||
| Average | 630,923 | 246,578 | 317,842 | 1,597,894 | 747,294 | 2,211,252 | 16,643,974 | 7,727,808 | 10,880,689 |
| SEM | 146,814 | 50,549 | 99,337 | 477,708 | 323,267 | 872,536 | 4,096,440 | 2,567,434 | 3,288,223 |
| Percent recovery | N/A | 39.1% | 50.4% | N/A | 46.8% | 138.4% | N/A | 46.4% | 65.4% |
The number of cells positive for each marker per gram of tissue isolated from fresh tissue cell isolation and recovered from either frozen cell or frozen tissue. Percent Recovery was calculated by dividing the average after cryopreservation by the average before cryopreservation. No significant differences were observed.