Table 1.
Oligonucleotides for HeV and NiV microsphere array assays.
| Name | Function | Sequence (5′-3′) | Position (a) |
|---|---|---|---|
| N-gene | |||
| D-358 | PCR-Fwd | TTTGAMGAGGCGGCTAGTTT | 125–144 |
| D-368 | PCR-Rev | CATCAARCCTTCCATCTCCTC | 499–479 |
| D-676 | TSPE-henipa (*MTAG-A057) | (*)-GCRGCAACWGCTACTTTGAC | 188–207 |
| D-679 | TSPE-HeV (*MTAG-A061) | (*)-ACTAATAGCCCAGAACTGAGATG | 236–258 |
| D-680 | TSPE-NiV (*MTAG-A067) | (*)-ACTAATAGTCCAGAGCTCAGATG | 236–258 |
| P-gene | |||
| D-550 | PCR-Fwd | ACATACAACTGGACCCARTGGTT | 2698–2720 |
| D-551 | PCR-Rev | CACCCTCTCTCAGGGCTTGA | 2794–2775 |
| D-641 | TSPE Henipa (*MTAG-A051) | (*)-ACAGACGTTGTATACCATG | 2721–2739 |
(a) Positions are relative to the HeV genome (GenBank accession number: NC_001906). TSPE denotes target-specific primer extension.
(*) denotes sequence of 3′ extension containing anti-TAG sequence complementary to TAG sequence on microsphere as defined by particular MTAG-A# (Luminex Corporation, USA).