Table 2.
Detection of HeV in experimentally infected horses.
| Day | Microsphere array assay | |||
|---|---|---|---|---|
| Henipa N | Henipa P | qPCR (a) | LAMP (a) | |
| (MFI) | (MFI) | (Ct) | (U/Pos) | |
| Horse 1 | ||||
| 0 | 148 | 213 | U | U |
| 1 | 185 | 256 | U | U |
| 2 | 20 858 | 23 745 | 37.5 | U |
| 3 | 22 088 | 24 593 | 34.7 | Pos |
| 4 | 22 828 | 24 049 | 35.9 | Pos |
| 5 | 23 734 | 24 985 | 29.5 | Pos |
| 6 | 23 856 | 25 197 | 32.8 | Pos |
|
| ||||
| Horse 2 | ||||
| 0 | 219 | 104 | U | U |
| 1 | 197 | 296 | U | Pos |
| 2 | 22 304 | 23 547 | 36.3 | Pos |
| 3 | 22 983 | 23 625 | 32.4 | Pos |
| 4 | 11 638 | 8 142 | 38.9 | Pos |
| 5 | 23 063 | 24 068 | 34.3 | Pos |
| 6 | 22 914 | 23 333 | 31.1 | Pos |
| 7 | 23 024 | 24 284 | 28.1 | Pos |
| 8 | 22 900 | 24 145 | 29.2 | Pos |
| 9 | 22 900 | 24 951 | 35.2 | Pos |
|
| ||||
| Horse 3 | ||||
| 0 | 186 | 301 | U | U |
| 1 | 221 | 288 | U | U |
| 2 | 10 734 | 375 | 42* | Pos |
| 4 | 1 271 | 2 251 | 41.4* | Pos |
| 5 | 5 406 | 7 018 | 43* | Pos |
| 6 | 1 804 | 8 019 | U | U |
| 7 | 16 731 | 20 575 | 37.7 | Pos |
|
| ||||
| Pos control | 23228 | 23451 | ||
| NTC | 226 | 298 | ||
Comparison of microsphere array assays performed on archival RNA extracted from daily nasal swabs of experimentally infected horses [21].
(a) Comparison with qPCR and loop-mediated amplification (LAMP) assay results [22] in retrospective analysis. Day indicates sampling day after challenge. MFI: median fluorescence intensity; U: undetected; Pos: positive reaction; *indicates qPCR indeterminate results; NTC: no template control. All positive results are in bold.