Table 3.
The average number of T. vaginalis parasites attached to HeLa cell monolayers per coverslip with or without treatment using different proteinase inhibitors (Figure 8(B)).
| Treatment | Parasites attached | Inhibition (%) |
|---|---|---|
| Nonea | 1450 | 0.0 ± 13.9176 |
| MA (5 μM)b | 742 | 46.33 ± 7.7764 |
| MA (10 μM) | 556 | 60.78 ± 5.6311 |
| MA (50 μM) | 301 | 77.23 ± 5.2907 |
| TLCK (1 mM) | 622 | 57.05 ± 6.6416 |
| Leupeptin (0.2 mM) | 898 | 36.79 ± 6.1359 |
| E-64 (0.18 mM) | 662 | 50.16 ± 8.1047 |
aNone corresponds to the control parasites without treatment with CP inhibitors. The number of parasites attached to the HeLa cell monolayer was taken as 100% adherence for comparative purpose. bMA corresponds to the Aza-Peptidyl Michael Acceptor, a legumain-specific inhibitor. These differences were statistically significant with a P < 0.001 (Figure 8(B)).