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. 2013 Feb 14;62(3):864–874. doi: 10.2337/db12-1089

FIG. 2.

FIG. 2.

Endogenous expression of Fabp4 in wild-type mice. A: Double immunofluorescence for the endothelial cell markers CD31 and Fabp4 in the perigonadal fat, heart, and muscle of 2-month-old C57BL/6 male mice. Perigonadal fat pads were whole-mount stained and representative composite (original magnification ×10) pictures created from Z-stacks of several images taken by confocal microscopy are shown (scale bar = 50 μm). Images for double immunofluorescence for CD31 and Fabp4 in heart and muscle are representative original magnification ×20 images (scale bar = 200 μm). B: Double immunofluorescence for the macrophage marker, F4/80 and Fabp4 in the perigonadal fat of wild-type mice. Representative images (original magnification ×40) of double immunofluorescence for F4/80 and Fabp4 in the perigonadal fat of 12-month-old wild-type C57BL/6 mice (scale bar = 50 μm). C: qPCR analysis of Fabp4 from sorted F4/80-positive cells from the peritoneal cavity and adipose tissue and whole adipose tissue of male mice (n = 6) after 12 weeks of a high-fat diet (started at age 6 weeks). Data are normalized to the expression of TATA binding protein (Tbp) and are shown as mean ± SEM.