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. 2013 Feb 14;62(3):864–874. doi: 10.2337/db12-1089

FIG. 3.

FIG. 3.

Analysis of Cre-induced Recombination in the aP2-CreERT2 R26R-lacZ mice. A: Whole-mount X-gal staining of fat pads from the aP2-CreERT2 R26R-lacZ mouse. Subcutaneous and perigonadal adipose tissue from 4 month old chow-fed aP2-CreERT2 R26R-lacZ (n = 3 per group) treated with vehicle or tamoxifen after overnight incubation in X-gal staining solution. B: Representative sections from aP2-CreERT2 R26R-lacZ X-gal-stained fat pads. Fat pads from Fig. 2A were fixed in Bouin’s fixative, embedded in paraffin, and 8-μm sections were stained with hematoxylin. Representative images are shown. C: Representative sections from aP2-CreERT2 R26R-lacZ tissues stained with X-gal. Heart, skeletal muscle, and salivary from 4-month-old chow-fed aP2-CreERT2 R26R-lacZ treated with vehicle or tamoxifen (n = 3) were embedded in optimal cutting temperature medium, and 10-μm sections were X-gal stained and counterstained with nuclear fast red. Representative images are shown.