FIG. 2.

Recruitment of IGRP_206–214-reactive CD8⁺ T cells from diabetic IGRP_206–214-competent or IGRP_206–214-deficient NOD hosts into islet grafts from IGRP_206–214-competent or IGRP_206–214-deficient donors. A: Percentages of NRP-V7/K^d tetramer-positive in islet-graft–associated CD8⁺ T cells. Data (average ± SEM) correspond, from left to right, to eight, four, four, and three grafts per group, respectively. B: Interferon-γ (IFN-γ) secretion by islet graft-associated CD8⁺ T cells in response to NRP-V7 peptide-pulsed NOD dendritic cells. Data correspond, from left to right, to five, four, four, and three grafts per group, respectively. C: Representative fluorescence-activated cell sorting staining profiles of CD8⁺ T cells isolated from islet grafts in the four different donor/host combinations. TUM/K^d was used as a negative control tetramer. P values in (A) and (B) were obtained with Mann-Whitney U test. Grafts were harvested immediately after the last blood glucose measurement in Fig. 1. (A high-quality color representation of this figure is available in the online issue.)