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. 2013 Feb 14;62(3):875–886. doi: 10.2337/db12-0952

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© 2013 by the American Diabetes Association.

Readers may use this article as long as the work is properly cited, the use is educational and not for profit, and the work is not altered. See http://creativecommons.org/licenses/by-nc-nd/3.0/ for details.

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FIG. 1. — Ldb1 is enriched in islet and ductal cells. A: Ldb1 (left) and Ldb2 (middle) mRNA expression was visualized by RNA ISH under identical conditions in E15.5 tissue. A higher-magnification view of pancreatic Ldb1 and Ldb2 expression is shown on the right. B: qPCR was performed to measure Ldb1, Ldb2, and Isl1 mRNA levels in E15.5 total pancreas (black bars) and 3-month-old isolated islets (gray bars). Expression levels are displayed relative to TATA-binding protein (TBP), which is set as onefold. Error bars represent ± SEM (n = 5). Ldb1 mRNA is significantly more abundant than Ldb2 in E15.5 and adult samples. C–K: Ldb1, Pdx1, Isl1, hormone (insulin, glucagon, and somatostatin), and ductal (DBA, CK-19) markers were visualized at E10.5, E18.5, P5, and P21 by coimmunofluorescence. Yellow dashed lines mark dorsal and ventral pancreas domains in C and D. Notably, only a few of the pancreatic Ldb1⁺ cells in D are copositive for Isl1 at this stage (some marked by white arrowheads). L: Immunohistochemical analysis illustrates enriched Ldb1 protein (brown) expression in adult human islet cells; the sample is eosin (pink) counterstained. *P < 0.05. D, dorsal pancreas; du, duct; G, gut tube; in, intestine; k, kidney; L, liver; M, mesenchyme; nt, neural tube; P, pancreas (outlined with red dashed line); st, stomach; V, ventral pancreas. (A high-quality digital representation of this figure is available in the online issue.)