FIG. 5.
Glp1r is a novel Ldb1- and Isl1-activated target gene. A: qPCR quantification of Isl1 ChIP-Seq candidates from Ldb1- (blue bars) and Isl1-deficient (red bars) E18.5 pancreata (n = 4–6). Data are presented as fold of the littermate control, which was set at 1 (marked by the dashed line), ± SEM. B and C: Immunostaining of Glp1r (brown) and insulin (red) at P6 illustrates reduced Glp1r protein levels in insulin+ cells lacking Ldb1 or Isl1. D: βTC-3 ChIP-Seq pictograph demonstrating the four distal 5′ peaks of Isl1 occupancy near Glp1r. The red line denotes the Glp1r locus. E: ChIP enrichment of peak 1 Glp1r 5′ DNA in Isl1 (top panel) and Ldb1 βTC-3 immunoprecipitates (bottom panel) as compared with IgG control-treated DNA. H2O serves as a negative control for the PCR. *P < 0.05. (A high-quality digital representation of this figure is available in the online issue.)