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. 2013 Feb 14;62(3):811–824. doi: 10.2337/db11-1652

FIG. 7.

FIG. 7.

Proteasome dysfunction upregulates FoxO1 protein amounts and gluconeogenic gene expression in the liver of PA28 KO mice. A: Total liver extracts and nuclear fractions from the livers of WT and PA28 KO mice were analyzed by Western blotting for phosphorylated and total FoxO1. GAPDH served as internal control. Data represent means ± SE (n = 4–5 per group). *P < 0.05. Nuclear fractions from in the livers of WT and PA28 KO mice were analyzed by Western blotting for total FoxO1. Lamin A/C GAPDH served as internal control. Data represent means ± SE (n = 5 per group). *P < 0.05. B and C: Relative mRNA levels of FoxO1, G6pc, Pck1, Igfbp1, and Ppargc1a in the liver of WT and PA28 KO mice were analyzed by RT-PCR. Data were normalized according to GAPDH levels. Data represent means ± SE (n = 7 per group). *P < 0.05.