FIG. 6.

Paroxetine reduces hyperglycemia-induced oxidative damage in endothelial cells. bEnd.3 cells were exposed to hyperglycemia (high) or maintained in medium containing normal glucose (low) for 10 days and treated with paroxetine (PRX; 10 μmol/L) or vehicle (CTL) for 3 days. Representative micrographs and respective bar graphs of the Comet assay (A, C); 8-hydroxy-guanosine (red) immunostaining with simultaneous Hoechst 33342 (blue) nuclear and phalloidin (green) actin staining (B) are shown. D: Crude mitochondrial fractions were prepared and processed with OxyBlot Protein Oxidation Detection Kit. Representative blot image of oxidized proteins and densitometric analysis are shown. *P < 0.05 compared with normoglycemic cells; #P < 0.05 compared with hyperglycemic cells.