Figure 4. Epitope spreading to PLP and MBP in MOG_35–55 immunized Daf1^−/− and Daf1^+/+ mice.

A) IFN-γ responses to PLP protein of MOG_35–55 immunized Daf1^−/− and Daf1^+/+ mice at day 56. In one Daf1^−/− mouse, low reactivity with splenocytes (detectable in the absence of PLP probably representing spontaneous blastogenesis) did not differ in the presence of Ova_323–339 control. B) Kinetics of T cell responses of MOG_35–55 immunized mice spreading to PLP. Ratio of Daf^−/− IFN-γ producing cells to Daf1^+/+ IFN-γ producing cells in response to the MOG_35–55 immunogen are shown on the left and spreading to PLP is shown on the right. C) IFN-γ recall responses in MOG_35–55 immunized mice at day 56 to PLP (50 µg/ml) and MBP (100 µg/ml).