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. Author manuscript; available in PMC: 2013 Feb 25.
Published in final edited form as: J Immunol. 2008 May 1;180(9):5882–5889. doi: 10.4049/jimmunol.180.9.5882

Figure 6. Augmented T cell responses by Daf deficient APCs and T cells depend on C5aR and C3aR.

Figure 6

A) IL-23p19 and IL-12p35 mRNA expression in sorted Daf1+/+, Daf1−/−, and Daf1−/−C3aR−/−C5aR−/− DCs after incubation with ova323–339 and OT-II cells. B). Western Blots of C3a and C5a in supernatants of Daf1+/+, Daf−/−, Daf1−/−C3aR−/− and Daf1−/−C5aR−/− T cells following anti-CD3 and anti-CD28 antibody stimulation. C) IFN-γ and IL-17 ELISPOT assays of Daf1+/+, Daf1−/−, Daf1−/− C5aR−/−, and Daf1−/−C3aR−/− T cells following anti-CD3 and anti-CD28 antibody stimulation. D) IFN-γ and IL-17 protein levels in supernatants of anti-CD3 and anti-CD28 antibody stimulated T cells from WT and relevant knockout mice. T cell were isolated by CD3+ T cell enrichment columns (R&D Systems).