Figure 7. Differentiation of naïve CD4⁺CD62L^hi T cells into IL-17 producing cells is augmented in the absence of Daf and markedly diminished in the absence of C5aR/C3aR and clinical scores are correspondingly decreased.

A) Generation of IL-17 producing cells. Sorted naïve (CD4⁺ CD62L^hi) T cells were stimulated with anti-CD3 (5µg/ml), anti-CD28 (10 µg/ml), anti-IFN-γ (10 µg/ml), and anti-IL-4 (10 µg/ml) together with TGF-β or TGF-β plus IL-6 for 72 hr. Supernatants were assayed for IL-17 protein by the Beadlyte mouse multicytokine detection system. Naïve T cells treated with mAbs only served as a control. B) More severe EAE in Daf1^−/− mice depends on C5aR and C3aR. Clinical scores of Daf1^−/− (n=5) and Daf1^+/+ (n=5), Daf1^−/−C3aR^−/− (n=3), and Daf1^−/−C5aR^−/− (n=3) mice measured daily after immunization with MOG_35–55.