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. Author manuscript; available in PMC: 2013 Feb 25.
Published in final edited form as: Methods Mol Biol. 2009;493:179–190. doi: 10.1007/978-1-59745-523-7_11

Table 11.1.

Primers utilized in the multilocus sequence typing of S. pneumoniae

Locus Primer Sequence (5′-3′)
aroE aroM13F
aroM13R		 TGTAAAACGACGGCCAGTGCCTTTGAGGCGACAGC
AGGAAACAGCTATGACCATTGCAGTTCARAAACATWTTCTAA
gdh gdhM13F
gdhM13R		 TGTAAAACGACGGCCAGTATGGACAAACCAGCNAGYTT
AGGAAACAGCTATGACCATGCTTGAGGTCCCATRCTNCC
gki gkiM13F
gkiM13R		 TGTAAAACGACGGCCAGTGGCATTGGAATGGGATCACC
AGGAAACAGCTATGACCATTCTCCCGCAGCTGACAC
recP recPM13F
recPM13R		 TGTAAAACGACGGCCAGTGCCAACTCAGGTCATCCAGG
AGGAAACAGCTATGACCATTGCAACCGTAGCATTGTAAC
spi spiM13F
spiM13R		 TGTAAAACGACGGCCAGTTTATTCCTCCTGATTCTGTC
AGGAAACAGCTATGACCATGTGATTGGCCAGAAGCGGAA
xpt xptM13F
xptM13R		 TGTAAAACGACGGCCAGTTTATTAGAAGAGCGCATCCT
AGGAAACAGCTATGACCATAGATCTGCCTCCTTAAATAC
ddl ddlM13F
ddlM13R		 TGTAAAACGACGGCCAGTTGCYCAAGTTCCTTATGTGG
AGGAAACAGCTATGACCATCACTGGGTRAAACCWGGCAT
M13 M13F
M13R		 TGTAAAACGACGGCCAGT
AGGAAAGACGTATGACCAT
*

Underlined sections represent M13 gene specific primer sequences (see Note 1).