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Intimomedial area of an atherosclerotic lesion of a WHHL rabbit without VSOP injection. Despite the presence of numerous calcifying microvesicles in the von Kossa stain (A), the microvesicles appear pink in the Prussian blue stain (B), indicating absence of stainable amounts of iron.
Abbreviations: VSOP, very small superparamagnetic iron oxide particles; WHHL, Watanabe heritable hyperlipidemic.
