Table S2.

Scoring system for morphologic and histopathologic analysis of vessel segments by microscopic field of view using ×200 magnification

Criterion	Stain	Measurements/scores
Integrity of media	Anti-SMA	0: no loss of SMC layers 1:<50% loss of media 2:>50% loss of media 3:<5 layers of media remaining
Intimal smooth muscle cell proliferation	Anti-SMA	0: no SMC in intima 1: up to 5 SMC layers in intima 2: 5–10 SMC layers in intima 3:>.10 SMC layers in intima
Microvesicles	Movat pentachrome	0: no blue bodies1: up to 10 blue bodies 2: up to 20 blue bodies 3:>20 blue bodies
Calcification	von Kossa (Alizarin)	0: no black–brown (purple) structures 1: up to 100 black–brown (purple) spots 2:>100 black–brown (purple) spots 3: confluent black–brown (purple) areas
Ferric iron	Prussian blue	0: no blue spots 1: up to 20 blue spots 2: up to 50 blue spots 3:>50 blue spots
Macrophages	Anti-RAM-11	0: no red-stained structures 1: up to 10 red foci 2: up to 20 red foci 3:>20 red foci
Angiogenesis	Anti-VEGF	0: no red-stained structures 1: up to 10 red foci 2: up to 20 red foci 3:>20 red foci

Abbreviations: RAM-11, rabbit macrophage clone RAM-11; SMA, smooth muscle cell actin; SMC, smooth muscle cell; VEGF, vascular endothelial growth factor.